phospho nf κb ser468  (Bioss)

Journal: Frontiers in Pharmacology

Article Title: Identification and evaluation of a pinocembrin analog as a TRPV1 inhibitor with analgesic properties in murine pain models

doi: 10.3389/fphar.2025.1585181

Figure Lengend Snippet: The effect of PINO on NF-κB and MAPK pathways. RAW264.7 cells were treated with CPZ (10 μM) or PINO (10 μM) for 1 h, and whole-cell lysates were prepared and immunoblotted using p-p65, p65, p-IκBα, IκBα, p-p38, p38, p-JNK, JNK, p-ERK1/2, ERK1/2, TRPV1, β-actin, GAPDH, and β-tubulin antibody (A) . Fold change was determined after normalization with β-actin, GAPDH, or β-tubulin, with the right panels illustrating the fold change relative to the control: p-p65/p65 (B) , p-IκBα/IκBα (C) , p-p38/p38 (D) , p-JNK/JNK (E) , p-ERK1/2/ERK1/2 (F) and TRPV1/β-actin (G) (n = 6). Data are presented as mean ± SD. Statistical analysis by one-way ANOVA with Bonferroni’s post hoc test.

Article Snippet: Phospho-NF-κB p65 (Ser468) antibody, NF-κB p65 antibody, Phospho-IκB Alpha (Ser32/36) antibody, IκB Alpha antibody, Phospho-p38 MAPK (Thr180/Tyr182) antibody, p38 MAPK antibody, Phospho-JNK (Tyr185) antibody, JNK antibody, Phospho-ERK1/2 (Thr202/Tyr204) antibody, ERK1/2 antibody, TRPV1 antibody, β-actin antibody, glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody, β-Tubulin and Goat Anti-Rabbit IgG (H + L) were purchased from Proteintech Group, Inc. (Wuhan, China).

Techniques: Control

Journal: Frontiers in Immunology

Article Title: Extracellular microvesicles from patients with Rheumatoid arthritis promote dendritic cell activation in vitro

doi: 10.3389/fimmu.2025.1532114

Figure Lengend Snippet: Analysis of MAPK and NF-κB activation in DCs stimulated with RA patient EMVs. DCs untreated or stimulated with LPS, RA patient EMVs and HD EMVs were lysed and analyzed by western blot to evaluate: (A) Phospho-p38 expression using rabbit anti-phospho-p38 Ab. (B) Phospho-ERK expression using rabbit anti-phospho-ERK1/2 Ab. (C) phospho-NF-κB-p65 expression using rabbit anti-phospho-NF-κB-p65 Ab. Anti-β-tubulin mAb or anti-β-actin mAb were used to evaluate loading controls. Samples from one representative RA patient and HD are shown. Densitometric phospho-proteins/total proteins ratios were calculated in all HDs and RA patients and are summarized by histograms in the right panels of the figure. Data are reported as mean (S.D.). Statistical analysis indicated: **p < 0.01; ***p < 0.001; ****p < 0.0001.

Article Snippet: The PVDF membranes containing the samples stimulated for 15 min were incubated with polyclonal rabbit anti-phospho-ERK1/2 or anti-phospho-p38 Abs (Cell Signaling, Inc. Danvers, MA, USA); alternatively, the PVDF membranes containing samples stimulated for 45 min were incubated with polyclonal rabbit anti-phospho NF-κB-p65 (Cell Signaling, Inc.).

Techniques: Activation Assay, Western Blot, Expressing